Establishment of HPLC fingerprints of Kangfuxin Liquid and determintion of six constituents / 中成药

AIM To establish the HPLC fingerprints of Kangfuxin Liquid (extract of Periplaneta americana L.) and to determine the contents of six constituents.METHODS The analysis of this drug was performed on a TOSOH TSK-GEL ODS column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.07％ acetic acid) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS There were twenty-four common peaks in the fingerprints of ten batches of samples (Ⅰ-Ⅹ) with the similarities of 0.932-0.993 (except for sample Ⅰ).Uracil,hypoxanthine,xanthine,inosine,protocatechuic acid and Cyclo (Gly-Tyr) showed good linear relationships within the ranges of 3.460-173.0,3.960-198.0,3.596-179.8,1.338-66.9,3.672-183.6 and 3.552-177.6 μg/mL,whose average recoveries (RSDS) were99.8％ (2.65％),98.0％ (2.55％),99.7％ (1.59％),100.7％ (2.80％),102.0％ (2.09％) and 99.6％ (1.88％),respectively.CONCLUSION This accurate,stable and simple method can be used for the quality control of Kangfuxin Liquid.

Chemical constituents from seeds of Ferula sinkiangensis / 中草药

Objective: To study the chemical constitutions from the seeds of Ferula sinkiangensis. Methods: Using different chromatographic methods to isolate and purify the compounds and to identify their structures by physicochemical properties and spectroscopic technology. Results: Thirty-two compounds were isolated and elucidated as butanedioic acid (1), variecolorquinonesa (2), β-sitosterol (3), stigmasterol-3-O-glucoside (4), 1-(3-ethylphenyl)-1,2-ethanediol (5), pinoresinol (6), (7,8-cis-8,8'-trans)- 2',4'-dihydroxyl-3,5-dimethoxy-lariciresinol (7), secoisolariciresinol (8), n-hexacosanol (9), isoquercitrin (10), quercetin (11), ferulic acid (12), stigmasterol (13), luteolin-7-O-β-D-gluronide (14), (R)-2'-hydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxyhexacosan-2-yl] nonadanamide (15), macrathoinf (16), 1,5-di-O-caffeoylquinic (17), arctiin (18), parvifoliolsg (19), 5-hydroxymethyl fufural (20), daucosterol (21), vanillic acid (22), sucrose (23), rutin (24), neoarctina (25), methyl chlorogenate (26), 5-O-caffeoylquinic (27), uridine (28), inosine (29), 7-hydroxy coumarin (30), chlorogenic acid (31), and caffeic acid (32). Conclusion: Compounds 2, 5-11, 14-20, 22, 24-29, 31, and 32 are isolated from the plants of Ferula L. for the first time.

A new bisabolane-type sesquiterpenoid from Coreopsis tinctoria / 中国中药杂志

To study the chemical constituents of the inflorescences of Coreopsis tinctoria from Xinjiang, isolation and purification of constituents were carried out by column chromatography on macroporous resin (D101) , MCI gel, MDS gel, silica gel, Sephadex LH-20 and semi-preparative HPLC. The structures of the compounds were identified by physicchemical properties and spectral data analysis. Fourteen compounds were isolated and identified as coretinterpenoid A (1), coretinphenol (2), quercetin (3), quercetin-3-O-β-glucopyranoside (4), luteolin (5), taxifolin (6), 7, 3', 5'-trihydroxyflavanone (7), isookanin (8), isookanin-7-O-β-D-glucopyranoside (9), 5, 7, 3', 5'-tetrahydroxyflavanone-7-O-β-D-glucopyranoside (10), butein (11), okanin (12), sulfuretin (13), and linocinnamarin (14). Compound 1 was a new isabolane-type sesquiterpenoid and compounds 4, 10 and 13 were isolated from this plant for the first time.

Chemical constituents of lateral roots of Aconitum carmichaelii Debx / 药学学报

In order to find the cardiotonic constituents of lateral roots of Aconitum carmichaelii Debx., the investigation was carried out. Silica gel column chromatography, Sephadex LH-20, medium-pressure MCI and reverse phase ODS column chromatography were used to separate the 90% EtOH extract of the lateral roots of Aconitum carmichaelii Debx. The structures of the isolated compounds have been identified by chemical properties and spectroscopic analyses. Ten compounds were isolated and their structures were elucidated as benzoic acid-5-hydroxy-2-benzoyl-amino methyl ester (1), honokiol (2), pinoresinol (3), salicylic acid (4), p-hydroxy-cinnamic acid (5), songorine (6), karakoline (7), mesaconitine (8), hypaconitine (9) and 14-benzoylhypaconitine (10), separetely. Compound 1 is a new compound and its structure has been established by NMR, HR-ESI-MS, UV, IR and X-Ray. Compound 2-5 are isolated from the lateral roots of Aconitum carmichaelii Debx. for the first time.

Study on chemical constituents from roots and rhizomes of Acorus tatarinowii / 中草药

Objective: To study the chemical constituents from the roots and rhizomes of Acorus tatarinowii. Methods: Using different chromatographic methods to isolate and purify the constituents of A. tatarinowii, and their structures were identified by physicochemical properties and spectroscopic technology. Results: Thirteen compounds were isolated and identified as fumaric acid (1), nicotinic acid (2), p-hydroxybenzonic acid (3), uracil (4), N-trans-feruloyltyramine (5), thymine (6), variecolorquinone A (7), butanedioic acid (8), tatarol (9), tataroside-12-β-D-glucoside (10), β-sitosterol (11), 2, 5-dimethoxy- benzoquinone (12), and 5-hydroxymethyl-2-furaldehyde (13). Conclusion: Compounds 1-7 are isolated from the plants in Acorus L. for the first time.